NFYA antibody
Catalogue Number: GTX48667-GTX
GTX48667 Image
GTX48667 Image
GTX48667 Image
GTX48667 Image
GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 µg/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
GTX48667 Image
CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4oC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.
GTX48667 Image
IHC-P analysis of human prostate tissue using GTX48667 NFYA antibody. Dilution : 10 µg/mL
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 37 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | nuclear transcription factor Y subunit alpha , CBF-A , CBF-B , HAP2 , NF-YA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH. |
| Application: | ELISA, IHC-P, WB, EMSA |
Description
Description: Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Additional Text
Gene ID
4800
Gene Name
NFYA
Uniprot ID
P23511
Molecular Weight
37
Purification
Unpurified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:2000-1:10000. IHC-P: 1:400. ELISA: 1:20000-1:100000. EMSA: 2 µL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48667 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations