IL27 antibody (HRP)
Catalogue Number: GTX48685-GTX
GTX48685 Image
GTX48685 Image
GTX48685 Image
Recombinant mouse IL27/p28 was loaded at 0.25 µg. The blot was blocked with 1% BSA in TBST for 30 min at RT. Blot was incubated with rabbit anti-Mouse IL-27/p28 (HRP) antibody in 1% BSA/TBST at 1:5,000 for 30 min at RT. Data was collected using Bio-Rad VersaDocR 4000 MP.
GTX48685 Image
WB analysis of recombinant mouse IL27 protein using GTX48685 IL27 antibody (HRP). Loading : 0.25 µg Dilution : 1:5000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Gentamicin sulfate |
| Molecular Weight: | 27 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Conjugated |
| Alias: | interleukin 27 , IL-27 , IL-27-A , IL-27p28 , IL27-A , Il30 , p28 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length recombinant mouse IL27/p28 protein. |
| Application: | ELISA, WB, IHC |
Description
Description: The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.
Additional Text
Uniprot ID
Q8K3I6
Gene Name
IL27
Gene ID
246779
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
27
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:5000. ELISA: 1:10000-1:50000. IHC: 1:1:500-1:2500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48685 Datasheet
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