C/EBP delta antibody
Catalogue Number: GTX48713-GTX
GTX48713 Image
GTX48713 Image
GTX48713 Image
GTX48713 Image
Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4oC Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
GTX48713 Image
WB analysis of transfected and non-transfected whole cell lysate using GTX48713 C/EBP delta antibody. Lane 1 : Human C/EBP beta Lane 2 : Mouse C/EBP delta Lane 3 : Mock Lane 4 : Human C/EBP delta Loading : 35 µg Dilution : 1:1500
GTX48713 Image
WB analysis of LPS-treated and untreated mouse primary macrophage lysate using GTX48713 C/EBP delta antibody. Lane 1 : Wild-type Lane 2 : Wild-type cells treated with LPS Lane 3 : C/EBP delta knockout cells treated with LPS Loading : 35 µg Dilution : 1:1500
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 29 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | CCAAT/enhancer binding protein (C/EBP), delta , c/EBPdelta |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to the amino terminus of mouse C/EBP. |
| Application: | ELISA, IP, WB, IHC-Flt |
Description
Description: C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBPd may increase binding activity, whereas phosphorylation of the a and b family members may decrease binding affinity. C/EBPd is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.
Additional Text
Gene Name
CEBPD
Purification
Affinity Purified
Gene ID
12609
Uniprot ID
Q00322
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
29
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:15000. IP: 0.8-1.5 µg/mL. ELISA: 1:4000-1:9000. IHC (Free Floating): 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48713 Datasheet
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