IL17F antibody
Catalogue Number: GTX48759-GTX
GTX48759 Image
GTX48759 Image
GTX48759 Image
Western blot using GeneTex's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F. Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 µg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4oC. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer) for 30 min at RT followed by reation with FemtoMax Chemiluminescent substrate. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.
GTX48759 Image
WB analysis of recombinant mouse IL17F protein using GTX48759 IL17F antibody. Loading : 0.25 µg Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 18 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interleukin 17F , C87042 , IL-17F |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length recombinant mouse IL17-F protein. |
| Application: | ELISA, WB, DB |
Description
Description: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Additional Text
Gene ID
257630
Gene Name
IL17F
Uniprot ID
Q7TNI7
Purification
Protein A purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
18
Concentration
1 mg/ml
Application Notes
WB: 1:1000. ELISA: 1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX48759 Datasheet
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