MED25 antibody, N-term
Catalogue Number: GTX49103-GTX
GTX49103 Image
GTX49103 Image
GTX49103 Image
ChIP analysis of HCT116 cells using GTX49103 MED25 antibody. Use 0.5µg antibody GTX49103 MED25 antibody for ChIP assay.Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site. (+) : Add blocking peptide (-) : no blocking peptide
GTX49103 Image
WB analysis of human heart tissue using GTX49103 MED25 antibody at 0.2-1µg/ml.
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 78 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | mediator complex subunit 25 , ACID1 , ARC92 , BVSYS , CMT2B2 , P78 , PTOV2 , TCBAP0758 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A synthetic peptide corresponding to a N-terminal region of Human MED25 |
| Application: | ChIP, WB |
Description
Description: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]
Additional Text
Gene Name
MED25
Uniprot ID
Q71SY5
Concentration
0.5-1 mg/ml
Purification
Affinity Purified
Gene ID
81857
Molecular Weight
78
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 0.2-2.5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX49103 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations