CD11b antibody [OX-42] (Biotin)
Catalogue Number: GTX53827-GTX
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Alias: | Integrin Subunit Alpha M , Cd11B , Itgam |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | OX-42 |
| Isotype: | IgG2a |
| Immunogen: | Rat peritoneal macrophages |
| Application: | FACS, IHC-Fr, IHC |
Description
Description: CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.GTX53827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.
Additional Text
Gene Name
ITGAM
Concentration
0.1 mg/ml
Purification
Protein G purified
Gene ID
25021
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
This antibody has been used in Immunohistochemistry (Frozen sections), Flow Cytometry / FACS. Recomended dilutions - FACS: Use neat - 1/10, or 10µl of the suggested working dilution to label 106 cells in 100µl. IHC-Fr: Use at an assay dependent dilution. Note: samples need to be PLP (periodate-lysine-paraformaldehyde) fixed. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
GTX53827 Datasheet
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