Lamin B1 antibody
Catalogue Number: GTX54288-GTX
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ChIP analysis of HeLa cell using GTX54288 Lamin B1 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR.
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ICC/IF analysis of PC-12 cells using GTX54288 Lamin B1 antibody. Dilution : 1:150 Blue : DAPI
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ICC/IF analysis of U2OS cells using GTX54288 Lamin B1 antibody. Dilution : 1:150 Blue : DAPI
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ICC/IF analysis of HeLa cells using GTX54288 Lamin B1 antibody. Dilution : 1:200 Blue : DAPI
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IHC-P analysis of mouse kidney tissue using GTX54288 Lamin B1 antibody. Antigen retrieval : 10 mM citrate buffer (pH 6.0) Dilution : 1:250
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IHC-P analysis of rat brain tissue using GTX54288 Lamin B1 antibody. Antigen retrieval : 10 mM citrate buffer (pH 6.0) Dilution : 1:250
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IHC-P analysis of human esophageal cancer tissue using GTX54288 Lamin B1 antibody. Antigen retrieval : 10 mM citrate buffer (pH 6.0) Dilution : 1:250
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IP analysis of HeLa cell lysates using GTX54288 Lamin B1 antibody. Total extraction : 300 µg IP antibody amount : 3µg Dilution : 1:1000
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WB analysis of various cell lysates using GTX54288 Lamin B1 antibody. L929 cells were treated by staurosporine(1 µM) for 3 hour. Loading: 25µg per lane Dilution : 1:1000
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WB analysis of various cell lysates using GTX54288 Lamin B1 antibody. Jurkat cells were treated by Etoposide (25 µM) at 37oC for 5 hours. Loading: 25µg per lane Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 66 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | lamin B1 , ADLD , LMN , LMN2 , LMNB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human LMNB1 (NP_005564.1). |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Additional Text
Gene Name
LMNB1
Uniprot ID
P20700
Gene ID
4001
Purification
Affinity Purified
Molecular Weight
66
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54288 Datasheet
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