CDC42 antibody
Catalogue Number: GTX54514-GTX
GTX54514 Image
GTX54514 Image
GTX54514 Image
GTX54514 Image
GTX54514 Image
GTX54514 Image
GTX54514 Image
IHC-P analysis of human pancreas tissue using GTX54514 CDC42 antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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WB analysis of 50 ug of various whole cell lysate using GTX54514 CDC42 antibody. Dilution : 1:1000
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ICC/IF analysis of HeLa cells using GTX54514 CDC42 antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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ICC/IF analysis of A431 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
GTX54514 Image
ICC/IF analysis of C6 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of HepG2 cells using GTX54514 CDC42 antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 21 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | cell division cycle 42 , CDC42Hs , G25K , TKS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | C-Terminal truncated human recombinant protein |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
Additional Text
Gene Name
CDC42
Uniprot ID
P60953
Gene ID
998
Purification
Protein A purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
21
Concentration
1 mg/ml
Application Notes
WB: 1:1000. ICC/IF: 1:100. IHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54514 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations