Vinculin (phospho Tyr100) antibody
Catalogue Number: GTX54517-GTX
GTX54517 Image
GTX54517 Image
GTX54517 Image
GTX54517 Image
WB analysis of COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA which were either untreated (Lane 1 and 6) or treated with vanadate for 24 hr (Lane 2-5 and 7-10) using GTX54517 Vinculin (phospho Tyr100) antibody (left panel) or Vinculin antibody (right panel).
GTX54517 Image
WB analysis of whole cell extracts (30 µg lysate) of HeLa (Lane 1) and HeLa treated with 100ng/ml of LPS for 20mins (Lane 2) using GTX54517 Vinculin (phospho Tyr100) antibody. Dilution : 1:1000
GTX54517 Image
ICC/IF analysis of HeLa cells using GTX54517 Vinculin (phospho Tyr100) antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minutes Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 124 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | vinculin , CMD1W , CMH15 , HEL114 , MV , MVCL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken. |
| Application: | FC, ICC, IF, WB |
Description
Description: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
VCL
Uniprot ID
P18206
Gene ID
7414
Purification
Affinity Purified
Molecular Weight
124
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Modification
Phosphorylated, Tyr100
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:1000. ICC/IF: 1:250. FCM: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX54517 Datasheet
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