GATA3 antibody
Catalogue Number: GTX54527-GTX
GTX54527 Image
GTX54527 Image
GTX54527 Image
GTX54527 Image
GTX54527 Image
GTX54527 Image
GTX54527 Image
IHC-P analysis of human tonsil tissue using GTX54527 GATA3 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
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WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX54527 GATA3 antibody. Dilution : 1:1000
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WB analysis of whole cell extracts (20 ug lysate) of SH-SY5Y (Lane 1), Jurkat (Lane 2) and K-562 (Lane 3) using GTX54527 GATA3 antibody. Dilution : 1:500-1:1500
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WB analysis of 20 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX54527 GATA3 antibody. Dilution : 1:1000
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ICC/IF analysis of MCF-7 cells using GTX54527 GATA3 antibody. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 1 µg/ml in 0.1% BSA incubated for 3 hours at room temperature
GTX54527 Image
ICC/IF analysis of MCF7 (left panel) and SH-SY5Y cells (right panel) using GTX54527 GATA3 antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 48 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GATA binding protein 3 , HDR , HDRS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified partial recombinant GATA3 protein expressed in E. coli |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Additional Text
Gene ID
2625
Gene Name
GATA3
Uniprot ID
P23771
Purification
Protein G purified
Molecular Weight
48
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:1500. ICC/IF: 1-2 µg/ml. IHC-P: 1:50-1:500. FCM: 3-5 µg/10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX54527 Datasheet
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