GATA4 antibody
Catalogue Number: GTX54530-GTX
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IHC-P analysis of human pancreas tissue using GTX54530 GATA4 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:200
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IHC-P analysis of human small intestine using GTX54530 GATA4 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:200
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IHC-P analysis of mouse pancreas tissue using GTX54530 GATA4 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:500
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FACS analysis of Hela cells using GTX54530 GATA4 antibody compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of HepG2 cells using GTX54530 GATA4 antibody compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of NIH/3T3 cells using GTX54530 GATA4 antibody compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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WB analysis of 40 ug of HeLa nuclear fraction (NF) (Lane 1), HEK293T control (Lane 2), and HEK293T GATA4 over-expression lysate (Lane 3) using GTX54530 GATA4 antibody. Dilution : 1:1000
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WB analysis of whole cell extracts (20 µg lysate) of SH-SY5Y (Lane 1) and Hep G2 (Lane 2) using GTX54530 GATA4 antibody. Dilution : 1:500-1:2000
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ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody. Green : Primary antibody Blue : cell nucleus Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for 1 hour at room temperature
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ICC/IF analysis of permeabilized embryoid body endoderm generated from human episomal iPSC using GTX54530 GATA4 antibody. Green : Primary antibody Blue : cell nucleus Dilution : 1:100 in 3% BSA/PBS blocking buffer overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
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ICC/IF analysis of HepG2 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX54530 Image
ICC/IF analysis of NIH-3T3 cells using GTX54530 GATA4 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 45 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GATA binding protein 4 , ASD2 , TACHD , TOF , VSD1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Pooled peptides: RKEGIQTRKRKPKNLNKSK and CKQDSWNSLVLADSHGDIITA |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Additional Text
Gene ID
2626
Gene Name
GATA4
Uniprot ID
P43694
Purification
Affinity Purified
Molecular Weight
45
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:2000. ICC/IF: 1:50-1:200. IHC-P: 1:100-1:1000. FCM: 3-5 µg/10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX54530 Datasheet
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