PAX5 antibody
Catalogue Number: GTX54532-GTX
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IHC-P analysis of human tonsil tissue using GTX54532 PAX5 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
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IHC-P analysis of mouse lymph node tissue using GTX54532 PAX5 antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
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IP analysis of Raji cell lysates using GTX54532 PAX5 antibody. IP reaction : 2µg antibody / 750µg lysate Dilution : 1:1000
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FACS analysis of permeabilized Ramos cells (red histogram) or PBS alone control(black histogram) using GTX54532 PAX5 antibody. Fixation : 4% formaldehyde Dilution : 1:50
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WB analysis of 75µg of whole cell lysates from PAX5-expressing B cell lymphoma-derived cell lines, negative control Jurkat cells using GTX54532 PAX5 antibody. Dilution : 1:1000
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ICC/IF analysis of Ramos and negative control Jurkat cells using GTX54532 PAX5 antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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The data was published in the journal Cancers (Basel) in 2020.PMID: 32650570
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ICC/IF analysis of Raji cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
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ICC/IF analysis of Ramos cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 42 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | paired box 5 , ALL3 , BSAP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified internal fragment of human recombinant PAX5 expressed in E. coli |
| Application: | FC, ICC, IF, IHC-P, IP, WB |
Description
Description: This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Additional Text
Gene ID
5079
Gene Name
PAX5
Molecular Weight
42
Uniprot ID
Q02548
Purification
Protein G purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:2000. ICC/IF: 1:20-1:200. IHC-P: 1:50-1:500. FCM: 1:50. IP: 2-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX54532 Datasheet
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