PAX7 antibody
Catalogue Number: GTX54533-GTX
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GTX54533 Image
GTX54533 Image
GTX54533 Image
GTX54533 Image
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IP analysis of HeLa cell lysates using GTX54533 PAX7 antibody. IP reaction : 3µg antibody / 500µg lysate Dilution : 1:1000
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WB analysis of 50 µg of various whole cell lysates using GTX54533 PAX7 antibody. Dilution : 1:1000
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WB analysis of 50µg of HeLa lysate overexpressing PAX7 (right lane) or empty vector control (left lane) using GTX54533 PAX7 antibody. Dilution : 1:1000
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ICC/IF analysis of MCF7 and HeLa cells using GTX54533 PAX7 antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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ICC/IF analysis of C2C12 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
GTX54533 Image
ICC/IF analysis of HeLa cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
GTX54533 Image
ICC/IF analysis of MCF-7 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 55 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | paired box 7 , HUP1 , PAX7B , RMS2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Purified internal fragment of human recombinant PAX7 expressed in E. coli. |
| Application: | ICC, IF, IP, WB |
Description
Description: This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
Additional Text
Gene Name
PAX7
Purification
Protein G purified
Molecular Weight
55
Gene ID
5081
Uniprot ID
P23759
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000 - 1:2000. ICC/IF: 1:20-1:200. IP: 3 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54533 Datasheet
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