eIF2B3 antibody [1H3]
Catalogue Number: GTX54741-GTX
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GTX54741 Image
GTX54741 Image
GTX54741 Image
GTX54741 Image
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IHC-P analysis of human breast tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
GTX54741 Image
IHC-P analysis of human uterus tissue using GTX54741 eIF2B3 antibody [1H3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
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IP analysis of U2OS cell lysates using GTX54741 eIF2B3 antibody [1H3]. IP reaction : 3µg antibody / 500µg lysate Dilution : 1:1000
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WB analysis of 80µg whole cell lysate using GTX54741 eIF2B3 antibody [1H3]. Dilution : 1:1000
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ICC/IF analysis of COS7 cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight
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ICC/IF analysis of HeLa cells using GTX54741 eIF2B3 antibody [1H3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | eukaryotic translation initiation factor 2B subunit gamma , EIF-2B , EIF2Bgamma |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 1H3 |
| Isotype: | IgG1 |
| Immunogen: | Full length human eIF2B3 produced in HEK293T cells. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Additional Text
Gene Name
EIF2B3
Uniprot ID
Q9NR50
Gene ID
8891
Molecular Weight
50
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000. ICC/IF: 1:10-1:100. IHC-P: 1:100-1:200. IP: 3 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54741 Datasheet
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