eIF1 antibody [2B9]
Catalogue Number: GTX54748-GTX
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WB analysis of whole cell extracts (30 µg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9]. Dilution : 1:500-1:2000
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WB analysis of 80µg whole cell lysate using GTX54748 eIF1 antibody [2B9]. Dilution : 1:1000
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ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
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ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX54748 Image
ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX54748 Image
ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 13 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | eukaryotic translation initiation factor 1 , A121 , EIF-1 , EIF1A , ISO1 , SUI1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 2B9 |
| Isotype: | IgG2a |
| Immunogen: | Full lengthhuman EIF1 protein produced in HEK293T cells. |
| Application: | ICC, IF, WB |
Additional Text
Gene Name
EIF1
Gene ID
10209
Purification
Protein A purified
Uniprot ID
P41567
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
13
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:2000. ICC/IF: 1-2 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54748 Datasheet
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