Orexin Receptor 1 antibody
Catalogue Number: GTX54847-GTX
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ICC/IF analysis of PFA-fixed human colon cancer cells using GTX54847 Orexin Receptor 1 antibody. Panel A : Primary antibody Panel B : Live view of the same field as in (A) Panel C : DNA dye Hoechst 33342 Dilution : 1:200
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IHC-Fr analysis of mouse septum tissue using GTX54847 Orexin Receptor 1 antibody. Cell nuclei (blue) are visualized with Hoechst 33342. Panel A : OX1R (green) appears in axonal processes (right-pointing triangles). Panel B : Parvalbumin (red) appears in septal neurons. Panel C : Merge of OX1R and parvalbumin suggests that orexinergic innervation covers the entire septal nucleus rather than restricted to individual neurons. Dilution : 1:50
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IHC-Fr analysis of rat brain tissue using GTX54847 Orexin Receptor 1 antibody. Red : Primary antibody Blue : Hoechst 33342 ??is used as a counterstain Dilution : 1:500
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IHC-P analysis of rat colon tissue using GTX54847 Orexin Receptor 1 antibody. Note that the stain (red-brown color) is highly specific for absorptive cells in the superior third of the intestinal glands. Immunolabeling was detected using DAB as the chromogen and hematoxilin as the counterstain. Dilution : 1:100
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WB analysis of mouse brain tissue lysate using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide. Dilution : 1:200
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WB analysis of rat brain lysate using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide. Dilution : 1:500
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WB analysis of Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) cell lysates using GTX54847 Orexin Receptor 1 antibody preincubated with or without immunogen peptide. Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | hypocretin receptor 1 , Hctr1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 (3rd intracellular loop) of rat OX1R (Accession : P56718). |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: neuropeptide receptor that binds orexin A; mediates orexin A modulation of the sleep-wake cycle [RGD, Feb 2006]
Additional Text
Gene Name
HCRTR1
Concentration
0.8 mg/ml
Uniprot ID
P56718
Purification
Affinity Purified
Molecular Weight
47
Gene ID
25593
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX54847 Datasheet
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DELIVERY
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