gamma 2 Actin antibody
Catalogue Number: GTX55849-GTX
GTX55849 Image
GTX55849 Image
GTX55849 Image
GTX55849 Image
ICC/IF analysis of formalin-fixed HeLa cells using GTX55849 gamma 2 Actin antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
GTX55849 Image
IHC-P analysis of formalin fixed human muscle tissue section using GTX55849 gamma 2 Actin antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
GTX55849 Image
WB analysis of DLD (A), HeLa (B), mouse brain (C), rat brain (D) whole cell lysates using GTX55849 gamma 2 Actin antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 42 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | actin gamma 2, smooth muscle , ACT , ACTA3 , ACTE , ACTL3 , ACTSG , VSCM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of gamma 2 Actin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]
Additional Text
Gene ID
72
Gene Name
ACTG2
Uniprot ID
P63267
Molecular Weight
42
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500 - 1:1000. ICC/IF: 1:100 - 1:500. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX55849 Datasheet
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