TLR4 antibody [76B357.1]
Catalogue Number: GTX57153-GTX
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FACS (Intracellular staining) analysis of Jurkat cells using GTX57153 TLR4 antibody [76B357.1]. Blue : Primary antibody Orange : isotype control Dilution : 2.5 µg/ml Fixation : 4% PFA Permibilization : 0.1% saponin
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WB analysis of (A) human (B) mouse, and (C) rat intestine tissue lysate using GTX57153 TLR4 antibody [76B357.1]. Dilution : 2 µg/ml(A) ; 6µg/ml(B and C)
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FACS analysis of human monocyte-derived dendritic cells using GTX57153 TLR4 antibody [76B357.1]. Blue : Primary antibody Green : negative control Black : unstained cells Dilution : 1:200
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FACS analysis of human peripheral blood mononuclear cell (PBMC) using GTX57153 TLR4 antibody [76B357.1]. Cells were co-stained with anti-CD14 antibody. CD14+ cells were gated for analysis. Left : Isotype control Right : Primary antibody Antibody amount : 1µg
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FACS analysis of mouse splenocytes using GTX57153 TLR4 antibody [76B357.1]. Red : Primary antibody Green : isotype control Shaded histogram : cell only Antibody amount : 0.5 µg/10^6 cells
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FACS analysis of THP-1 cells using GTX57153 TLR4 antibody [76B357.1]. Blue : Isotype control Green : Primary antibody Antibody amount : 2 µg10^6 cells
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IHC-P analysis of rat salivary gland tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 1:100
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IHC-P analysis of human breast tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml
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IHC-P analysis of human colon tissue using GTX57153 TLR4 antibody [76B357.1]. TOP : isotype control Bottom : primary antibody Dilution : 5 µg/ml
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IHC-P analysis of human esophageal squamous cell carcinoma (SCC) tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml
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IHC-P analysis of human skin tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml
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IHC-P analysis of human skin tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
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IHC-P analysis of human small intestine malignant stromal tumor tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml
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IHC-P analysis of human testis tissue using GTX57153 TLR4 antibody [76B357.1]. Dilution : 5 µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | ChIP Validated Antibodies |
| Alias: | toll like receptor 4 , ARMD10 , CD284 , TLR-4 , TOLL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 76B357.1 |
| Isotype: | IgG2b |
| Immunogen: | This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564). |
| Application: | FACS, ICC, IF, IHC-P, ChIP, WB, IHC-Fr, MS, InVitroA |
Description
Description: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Also, several transcript variants of this gene have been found, but the protein coding potential of most of them is uncertain. [provided by RefSeq]
Additional Text
Gene Name
TLR4
Concentration
1 mg/ml
Gene ID
7099
Uniprot ID
O00206
Purification
Protein G purified
Molecular Weight
96
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1 - 3 µg/ml. ICC/IF: 1:10 - 1:500. IHC-P: 5 µg/ml. IHC-Fr: 1:500. ChIP assay: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX57153 Datasheet
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