SOD1 (mutant) antibody [MS785]
Catalogue Number: GTX57211-GTX
GTX57211 Image
GTX57211 Image
GTX57211 Image
GTX57211 Image
IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody's epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in "Appendix". Antibody amount : MS27 (GTX57212): 2µg, MS785 (GTX57211): 5µg, or MS27/MS785 cocktail: 1µg IP lysis buffer: 1% Triton X100/TBS buffer.
GTX57211 Image
IP analysis of HEK293 cells cultured in the presence and absence of 10µM TPEN (a potent zinc-specific chelator, for 8 hours) using GTX57211 SOD1 (mutant) antibody [MS785]. Under the zinc-deficient ER-stress, these antibodies could recognize endogenous SOD1 wild type with mutant like conformation. Antibody amount : 1-5 µg
GTX57211 Image
ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.2% Triton X-100 for 5 min Dilution : 1µg/ml (12 hours at 4oC)
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Molecular Weight: | 16 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Superoxide Dismutase 1 , Als , Als1 , Hel-S-44 , Ipoa , Sod , Hsod1 , Homodimer , Sod1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Rat |
| Clone: | MS785 |
| Isotype: | IgG2b |
| Immunogen: | Human SOD1 8-14 amino acids. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, IHC |
Additional Text
Gene Name
SOD1
Gene ID
6647
Concentration
0.5 mg/ml
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
16
Application Notes
ICC/IF: 1 µg/ml (1/500 dilution) -5 µg/ml (1/100 dilution). IP: 1-5µg/sample. IHC: 1 µg/ml (1/500 dilution) -5 µg/ml (1/100 dilution). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX57211 Datasheet
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