lacto-N-fucopentaose I antibody [R-17F]
Catalogue Number: GTX57213-GTX
GTX57213 Image
GTX57213 Image
GTX57213 Image
GTX57213 Image
GTX57213 Image
GTX57213 Image
Cultured human iPS cells were stained with R-17F, SSEA-3, and SSEA-4 antibodies. [bars: 100µm] R-17F stained the entire surface of the cell membranes equally, while the staining by SSEA-3 and SSEA-4 antibodies are not evenly. This suggests that R-17F epitope is expressed ubiquitously all over the human iPS cells.
GTX57213 Image
WB analysis of human iPS and HEK293 cell lysate using GTX57213 lacto-N-fucopentaose I antibody [R-17F]. One major positive band and several minor bands were specific to human iPS cells, and any positive band was not obtained with HEK293 cells. Human iPS cell: LNFP I positive HEK293 cell lysate: Negative Control Loading : 5ug per lane Dilution : 1:2000
GTX57213 Image
R-17F is reported to exhibit potent dose-dependent cytotoxicity when it is added to living human iPS/ES cells. [Left] After the incubation of iPS cell suspension with R-17F at 4oC for only 45 minutes, the percentage of viable cells decreased concentration-dependently (red triangles). Blue squares : effects of the isotype (IgG1)-matching control antibody (anti-α-MBP) as Negative Control. [Right] When R-17F-treated iPS cells were incubated with a small amount (0.025-0.1 µg) of the secondary antibody (goat anti-mouse IgG1 antibody), the cytotoxic effect of R-17F was enhanced significantly in a dose-dependent manner (red triangles).
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | R-17F |
| Isotype: | IgG1 |
| Immunogen: | Human iPS cell line, Tic, derived from human fetus lung cells. |
| Application: | FC, ICC, IF, WB, FA |
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX57213 Datasheet
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