Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade
Catalogue Number: GTX60327-GTX
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Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. Dilution : 1:1,000
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ELISA analysis of peptide containing the histone modification of interest using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade.
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ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:500
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WB analysis of 15 µg of histone extracts of HeLa cells treated with colcemid (to block the cell cycle in metaphase) using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. Dilution : 1:500
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ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60327 Histone H3K9me3S10ph (Tri-methyl Lys9/phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 µl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and c-fos and for the heterochromatin marker Sat2. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Histone H3 containing trimethylated lysine 9 and the phosphorylated serine 10 (H3K9me3S10p), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, ChIP, WB, DB |
Additional Text
Gene ID
8350
Purification
Unpurified
Modification
Phosphorylated, Ser10; Trimethylated, Lys9
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H3C1
Application Notes
WB: 1:500. ICC/IF: 1:500. Dot: 1:1000. ELISA: 1:1,000 - 1:10,000. ChIP assay: 5 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60327 Datasheet
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