Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody
Catalogue Number: GTX60328-GTX
GTX60328 Image
GTX60328 Image
GTX60328 Image
GTX60328 Image
GTX60328 Image
GTX60328 Image
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. Dilution : 1:20,000
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ELISA analysis of peptide containing the histone modifications of interest using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
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ICC/IF analysis of HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
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WB analysis of histone extracts (15 µg) from HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. Dilution : 1:250
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ChIP analysis of sheared chromatin from 1.5x10? U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 µl per ChIP experiment was analysed. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Histone H3 containing the acetylated lysine 9 and the phosphorylated serine 10 (H3K9acS10p), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, ChIP, WB, DB |
Additional Text
Gene ID
8350
Purification
Unpurified
Modification
Acetylated, Lys9; Phosphorylated, Ser10
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H3C1
Application Notes
WB: 1:250. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000 - 1:4,000. ChIP assay: 15 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60328 Datasheet
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