Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade
Catalogue Number: GTX60329-GTX
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Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3 using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. Dilution : 1:20,000
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ELISA analysis of peptide containing the histone modifications of interest using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade.
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ICC/IF analysis of paraformaldehyde fixed HeLa asynchronous cells using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. Phosphorylation of H3 on serine 28 is increased during late G2 phase and reaches a maximum in metaphase cells. This may explain the different staining intensities of different cells. Green : Primary antibody Blue : DAPI Dilution : 1:200
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WB analysis of histone extracts (15 µg) from HeLa cells using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. Dilution : 1:250
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ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 µl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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IP analysis of HeLa cells were treated with colcemid (to block the cell cycle in metaphase) using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. IP reaction : 5 µl antibody / 1x10? cells Lane 1 : Primary antibody Lane 2 : No antibody control Lane 3 : Input Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, IP, ChIP, WB, DB |
Additional Text
Gene ID
8350
Purification
Unpurified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Modification
Phosphorylated, Ser28; Trimethylated, Lys27
Gene Name
H3C1
Application Notes
WB: 1:250 - 1:500. ICC/IF: 1:200. IP: 5 µl. Dot: 1:20,000. ELISA: 1:100 - 1:500. ChIP assay: 1 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60329 Datasheet
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