Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade
Catalogue Number: GTX60334-GTX
GTX60334 Image
GTX60334 Image
GTX60334 Image
GTX60334 Image
GTX60334 Image
GTX60334 Image
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H4 using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade. Dilution : 1:20,000
GTX60334 Image
ELISA analysis of peptide containing the histone modification of interest using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade.
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ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:500
GTX60334 Image
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
GTX60334 Image
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60334 Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the signal distribution along the complete length of chromosome 5 (figure 2A) and a zoomin to a 600 kb region (figure 2B). Figure 2C and D show the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls. The position of the amplicon used for validating the QPCR results is shown with an arrow Antibody amount : 2µg
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The region of histone H4 containing the acetylated lysines 5, 8, 12 and 16 (H4K5,8,12,16ac), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, ChIP, DB |
Additional Text
Concentration
0.76 mg/ml
Purification
Affinity Purified
Gene ID
8359
Modification
Acetylated, Lys5
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H4C1
Application Notes
ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000. ChIP assay: 1-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60334 Datasheet
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