Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade
Catalogue Number: GTX60353-GTX
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ChIP analysis of HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1 Mb region of the X-chromosome (figure 2A and B), in 100 kb regions surrounding the GAPDH positive control and EIF4A2 genes (figure 2C and D). Antibody amount : 1µg
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Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K79 using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. Dilution : 1:20,000
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ELISA analysis of peptide containing the histone modification of interest using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
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WB analysis of whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H3 (lane 3) using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. Dilution : 1:200
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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Histone H3 containing the monomethylated lysine 79 (H3K79me1), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ChIP, WB, DB |
Additional Text
Gene ID
8350
Concentration
1.66 mg/mL
Purification
Affinity Purified
Modification
Monomethylated, Lys79
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H3C1
Application Notes
WB: 1:200. Dot: 1:20,000. ELISA: 1:500. ChIP assay: 1-2 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60353 Datasheet
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