MECP2 antibody - ChIP grade
Catalogue Number: GTX60358-GTX
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ELISA analysis of peptides used for immunization using GTX60358 MECP2 antibody - ChIP grade.
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WB analysis of nuclear extracts (25 µg) from HeLa cells using GTX60358 MECP2 antibody - ChIP grade. Dilution : 1:1,000
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WB analysis of whole cell extracts (40 µg) from HeLa cells transfected with MeCP2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60358 MECP2 antibody - ChIP grade. Dilution : 1:1,000
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ChIP analysis of sheared chromatin from 10? U2OS cells using GTX60358 MECP2 antibody - ChIP grade. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Molecular Weight: | 52 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | methyl-CpG binding protein 2 , AUTSX3 , MRX16 , MRX79 , MRXS13 , MRXSL , PPMX , RS , RTS , RTT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein. |
| Application: | ELISA, ChIP, WB |
Description
Description: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of cognitive disability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
Additional Text
Gene Name
MECP2
Gene ID
4204
Concentration
1.2 mg/mL
Purification
Affinity Purified
Molecular Weight
52
Uniprot ID
P51608
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1,000. ELISA: 1:1,000. ChIP assay: 1-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60358 Datasheet
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