Argonautes antibody [GT9003]
Catalogue Number: GTX60370-GTX
GTX60370 Image
GTX60370 Image
GTX60370 Image
GTX60370 Image
GTX60370 Image
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60370 Argonautes antibody [GT9003]. Red : Primary antibody Blue : DAPI Dilution : 1:500
GTX60370 Image
IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 µl of input (10% of starting material), 10 µl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded. Antibody amount : 4 or 12µg.
GTX60370 Image
RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target "A" allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target "G" allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).
GTX60370 Image
WB analysis of whole cell extracts (40 µg) from HeLa cells using GTX60370 Argonautes antibody [GT9003]. Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 97 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | argonaute RISC catalytic component 2 , CASC7 , EIF2C2 , LINC00980 , PPD , Q10 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | GT9003 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human Ago2 protein (GST fusion protein containing amino acids 47-879 of Ago2). |
| Application: | ICC, IF, IP, WB, IHC, RIP |
Description
Description: This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Additional Text
Uniprot ID
Q9UKV8
Gene ID
27161
Concentration
2.4 mg/mL
Purification
Protein A purified
Gene Name
AGO2
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
97
Application Notes
WB: 1:500. ICC/IF: 1:500. IP: 8 µg per IP. IHC: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60370 Datasheet
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