AMPK alpha 1 antibody [2B7]
Catalogue Number: GTX60403-GTX
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GTX60403 Image
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GTX60403 Image
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ELISA analysis of antigen using GTX60403 AMPK alpha 1 antibody [2B7]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
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FACS analysis of PC-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Purple : negative control
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ICC/IF analysis of NTERA-2 cells using GTX60403 AMPK alpha 1 antibody [2B7]. Green : AMPK alpha 1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
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IHC-P analysis of ovarian cancer (left) and brain tissue (right) using GTX60403 AMPK alpha 1 antibody [2B7].
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WB analysis of Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH-3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate using GTX60403 AMPK alpha 1 antibody [2B7].
| Manufacturer: | GeneTex |
| Preservative: | 0.03% Sodium azide |
| Molecular Weight: | 64 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | protein kinase AMP-activated catalytic subunit alpha 1 , AMPK , AMPKa1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 2B7 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human PRKAA1 expressed in E. Coli. |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Additional Text
Gene ID
5562
Uniprot ID
Q13131
Gene Name
Prkaa1
Molecular Weight
64
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX60403 Datasheet
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