eIF4E antibody [5D11]
Catalogue Number: GTX60420-GTX
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ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
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FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Purple : negative control
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ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
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IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].
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IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].
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WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].
| Manufacturer: | GeneTex |
| Preservative: | 0.03% Sodium azide |
| Molecular Weight: | 25 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 5D11 |
| Isotype: | IgG1 |
| Immunogen: | Purified recombinant fragment of human EIF4E expressed in E. Coli. |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB |
Description
Description: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Additional Text
Gene ID
1977
Gene Name
EIF4E
Uniprot ID
P06730
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
25
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX60420 Datasheet
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