ASK1 antibody [2E4]

Catalogue Number: GTX60456-GTX

Manufacturer:	GeneTex
Preservative:	0.03% Sodium azide
Molecular Weight:	155
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	2E4
Isotype:	IgG1
Immunogen:	Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Application:	ELISA, FC, ICC, IF, IHC-P, WB

Overview
Datasheets

Description

Description: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Additional Text

Gene Name

MAP3K5

Uniprot ID

Q99683

Gene ID

4217

Molecular Weight

155

Purification

Unpurified

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Application Notes

WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

GTX60456 Datasheet

Price

£410.00

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