NFkB p105/p50 antibody [5D10D11]
Catalogue Number: GTX60465-GTX
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GTX60465 Image
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ELISA analysis of antigen using GTX60465 NFkB p105/p50 antibody [5D10D11]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
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FACS analysis of MCF-7 cells using GTX60465 NFkB p105/p50 antibody [5D10D11]. Green : NFkB p105/p50 Purple : negative control
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ICC/IF analysis of U251 cells using GTX60465 NFkB p105/p50 antibody [5D10D11]. Green : NFkB p105/p50 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
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IHC-P analysis of human intima cancer tissue (left) and human bladder cancer tissue (right) using GTX60465 NFkB p105/p50 antibody [5D10D11].
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WB analysis of K562 (1), Jurkat (2), A431 (3), HeLa (4), THP-1 (5) and MCF-7 (6) cell lysate using GTX60465 NFkB p105/p50 antibody [5D10D11].
| Manufacturer: | GeneTex |
| Preservative: | 0.03% Sodium azide |
| Molecular Weight: | 105 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | nuclear factor kappa B subunit 1 , CVID12 , EBP-1 , KBF1 , NF-kB1 , NF-kappa-B1 , NF-kappaB , NFKB-p105 , NFKB-p50 , NFkappaB , p105 , p50 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 5D10D11 |
| Isotype: | IgG2a |
| Immunogen: | Purified recombinant fragment of human NFkB1 expressed in E. Coli. |
| Application: | ELISA, FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Additional Text
Gene Name
NFKB1
Gene ID
4790
Uniprot ID
P19838
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
105
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FCM: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX60465 Datasheet
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