5-Methylcytosine / 5-mC antibody [GT9076]
Catalogue Number: GTX60803-GTX
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Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Dilution : 1:600
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FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 µg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000
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ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000
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ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained. Left : Primary antibody Right : Anti- single stranded DNA antibody Permeabilization : 0.5% Triton X-100 for 30 mins Dilution : 1:200
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ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 µM mCTP (left) or with DAPI (right). Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 µM hmCTP and with DAPI. Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | GT9076 |
| Isotype: | IgG1 |
| Immunogen: | 5-mC (5-methylcytosine) conjugated to BSA. |
| Application: | ICC, IF, DB, FISH, MeDIP |
Additional Text
Concentration
1.3 mg/ml
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
ICC/IF: 1:1,000. Dot: 1:600. FISH: 1:200 - 1:1,000. MeDIP: 1-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60803 Datasheet
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