EZH2 antibody - ChIP grade
Catalogue Number: GTX60811-GTX
GTX60811 Image
GTX60811 Image
GTX60811 Image
GTX60811 Image
GTX60811 Image
GTX60811 Image
ChIP analysis of sheared chromatin from 4 million K562 cells using GTX60811 EZH2 antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure 2A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure 2C and D). Antibody amount : 2µg
GTX60811 Image
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60811 EZH2 antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:1000
GTX60811 Image
WB analysis of nuclear extracts (40 µg) from HeLa cells using GTX60811 EZH2 antibody - ChIP grade. Dilution : 1:1,000
GTX60811 Image
WB analysis of whole cell extracts (40 µg) from HeLa cells transfected with EZH2 siRNA (lane 2) and from an untransfected control (lane 1) using GTX60811 EZH2 antibody - ChIP grade. Dilution : 1:1,000
GTX60811 Image
ChIP analysis of sheared chromatin from 4x10? K562 cells using GTX60811 EZH2 antibody - ChIP grade. A titration of the antibody consisting of 1, 2. 5 and 10 µg per ChIP experiment was analysed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | enhancer of zeste 2 polycomb repressive complex 2 subunit , Enx-1 , Enx1h , KMT6 , mKIAA4065 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2). |
| Application: | ICC, IF, ChIP, WB, RIP |
Additional Text
Gene Name
EZH2
Uniprot ID
Q61188
Purification
Protein G purified
Molecular Weight
85
Gene ID
14056
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60811 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations