Histone H2A.Z antibody - ChIP grade
Catalogue Number: GTX60812-GTX
GTX60812 Image
GTX60812 Image
GTX60812 Image
GTX60812 Image
GTX60812 Image
GTX60812 Image
ELISA analysis of peptide containing the histone modification of interest using GTX60812 Histone H2A.Z antibody - ChIP grade.
GTX60812 Image
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. Staining of the cells with the H2A.Z antibody after incubation of the antibody with 10 ng/µl of the immunogen peptide (figure 5B) and with a peptide containing a sequence from the central part of the H2A.Z protein (figure 5C). Green : Primary antibody Blue : DAPI Dilution : 1:500
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WB analysis of whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 5, 6, 7 and 8, respectively) using GTX60812 Histone H2A.Z antibody - ChIP grade. Dilution : 1:1,000
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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
GTX60812 Image
ChIP analysis of sheared chromatin from 1x10? K562 cells using GTX60812 Histone H2A.Z antibody - ChIP grade. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome (hg19) using the ELAND algorithm. Figure 2 shows the peak distribution in four genomic regions including the regions surrounding the EIF2S3 and c-fos positive control genes on chromosome X and 14, respectively (figure 2A and B). Antibody amount : 0.5µg
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H2A histone family member Z , H2A histone family member V , H2A.Z-1 , H2A.z , H2A/z , H2AZ , H2A.Z-2 , H2AV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antibody was raised in rabbit against histone variant H2A. |
| Application: | ELISA, ICC, IF, ChIP, WB |
Additional Text
Concentration
1.55 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Uniprot ID
P0C0S5, Q71UI9
Application Notes
WB: 1:1,000. ICC/IF: 1:500. ELISA: 1:5,000. ChIP assay: 0.5-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Gene ID
3015; 94239
Gene Name
H2AZ1, H2AZ2
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60812 Datasheet
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