Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade
Catalogue Number: GTX60813-GTX
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Dot blot analysis of 0.2 - 100 pmol of the peptides containing different multiple acetylations and the unmodified H2A.Z using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. Dilution : 1:20,000
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ELISA analysis of peptide containing the histone modifications of interest using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade.
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ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:500
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WB analysis of whole cell extracts (25 µg, lane 1) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. Dilution : 1:1,000
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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes CCT5 and EIF4A2, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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ChIP analysis of sheared chromatin from 1x10? K562 cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the X-chromosome (figure 2A and B) and in two regions surrounding the EIF4A2 and CCT5 positive control genes, respectively (figure 2C and D). Antibody amount : 1µg
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | H2A histone family member Z , H2A histone family member V , H2A.Z-1 , H2A.z , H2A/z , H2AZ , H2A.Z-2 , H2AV |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The region of histone H2A.Z containing the acetylated lysines 4, 7 and 11, using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, ChIP, WB, DB |
Additional Text
Purification
Affinity Purified
Modification
Acetylated, Lys4
Concentration
1.09 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Uniprot ID
P0C0S5, Q71UI9
Gene ID
3015; 94239
Gene Name
H2AZ1, H2AZ2
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60813 Datasheet
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