Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade
Catalogue Number: GTX60825-GTX
GTX60825 Image
GTX60825 Image
GTX60825 Image
GTX60825 Image
GTX60825 Image
GTX60825 Image
GTX60825 Image
ChIP analysis of HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 500 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively). Antibody amount : 1µg
GTX60825 Image
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3R17K18 using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. Dilution : 1:20,000
GTX60825 Image
ELISA analysis of peptide containing the histone modification of interest using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade.
GTX60825 Image
ICC/IF analysis of 4% paraformaldehyde fixed NIH3T3 cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:500
GTX60825 Image
WB analysis of whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. Dilution : 1:1,000
GTX60825 Image
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60825 Histone H3R17me2K18ac (Asymmetric Di-methyl Arg17/Acetyl Lys18) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The region of histone H3 containing the asymmetrically dimethylated R17 and the acetylated lysine 18 (H3R17me2(asym)K18ac), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, ChIP, WB, DB |
Additional Text
Gene ID
8350
Concentration
0.94 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Modification
Acetylated, Lys18; Dimethylated, Asymmetric, Arg17
Gene Name
H3C1
Application Notes
WB: 1:1,000. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000. ChIP assay: 1-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60825 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations