Histone H3S10ph (phospho Ser10) antibody - ChIP grade
Catalogue Number: GTX60827-GTX
GTX60827 Image
GTX60827 Image
GTX60827 Image
GTX60827 Image
GTX60827 Image
GTX60827 Image
GTX60827 Image
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and with peptides containing unmodified sequences from histone H3 using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. Dilution : 1:20,000
GTX60827 Image
ELISA analysis of peptide containing the histone modification of interest using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade.
GTX60827 Image
ICC/IF analysis of paraformaldehyde fixed HeLa asynchronous cells using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. Phosphorylation of H3 on serine 10 occurs on condensed chromosomes during mitosis. This explains the dense staining of one of the cells (indicated with an arrow). Green : Primary antibody Blue : DAPI Dilution : 1:200
GTX60827 Image
WB analysis of 15 µg of histone extracts of HeLa cells treated with TSA (figure 4A) or with colcemid (figure 4B) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. Dilution : 1:500
GTX60827 Image
ChIP analysis of sheared chromatin from 10? HeLa cells treated with colcemid using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 µl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 µg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes c-fos and RPL30. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
GTX60827 Image
IP analysis of HeLa cells were treated with colcemid (to block the cell cycle in metaphase) using GTX60827 Histone H3S10ph (phospho Ser10) antibody - ChIP grade. IP reaction : 5 µl antibody / 1x10? cells Lane 1 : Primary antibody Lane 2 : Input Lane 3 : No antibody control Dilution : 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Histone H3 containing the phosphorylated serine 10 (H3S10p), using a KLH-conjugated synthetic peptide. |
| Application: | ELISA, ICC, IF, IP, ChIP, WB, DB |
Additional Text
Gene ID
8350
Modification
Phosphorylated, Ser10
Purification
Unpurified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H3C1
Application Notes
WB: 1:500. ICC/IF: 1:200. IP: 5 µl. Dot: 1:20,000. ELISA: 1:1,000 - 1:5,000. ChIP assay: 1-10 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60827 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations