HDAC3 antibody [GT9080]
Catalogue Number: GTX60832-GTX
GTX60832 Image
GTX60832 Image
GTX60832 Image
ChIP was performed with HeLa extracts and either 5 µg of HDAC3 antibody or control IgG. The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter, c-fos promoter and the coding region of p21, a known target gene of HDAC3.
GTX60832 Image
ICC/IF analysis of HeLa cells using HDAC3 antibody (red) and DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC3 antibody at a dilution of 1:500.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 49 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Mouse |
| Clone: | GT9080 |
| Isotype: | IgG |
| Immunogen: | Human HDAC3 (Histone deacetylase 3), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein. |
| Application: | ICC, IF, ChIP |
Description
Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
HDAC3
Gene ID
8841
Uniprot ID
O15379
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60832 Datasheet
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