pan-HP1 antibody - ChIP grade
Catalogue Number: GTX60833-GTX
GTX60833 Image
GTX60833 Image
GTX60833 Image
GTX60833 Image
ChIP analysis of NIH3T3 cells using GTX60833 pan-HP1 antibody - ChIP grade. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. This figure shows the relative enrichment as compared to a no antibody negative control ChIP. Antibody amount : 4µg
GTX60833 Image
ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade. Green : Primary antibody Blue : DAPI Dilution : 1:500
GTX60833 Image
WB analysis of nuclear extracts (20 µg) from HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade. Dilution : 1:1,000
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% ProClin 300|0.05% Sodium azide, 0.05% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | chromobox 1 , chromobox 3 , chromobox 5 , CBX , HP1-BETA , HP1Hs-beta , HP1Hsbeta , M31 , MOD1 , p25beta , HECH , HP1-GAMMA , HP1Hs-gamma , HEL25 , HP1 , HP1A |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms. |
| Application: | ICC, IF, ChIP, WB |
Additional Text
Concentration
2 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Uniprot ID
P45973, P83916, Q13185
Application Notes
WB: 1:1,000. ICC/IF: 1:500. ChIP assay: 4 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Gene ID
10951; 11335; 23468
Gene Name
CBX1, CBX3, CBX5
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60833 Datasheet
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