Histone H3K14ac (Acetyl Lys14) antibody [RM130]
Catalogue Number: GTX60874-GTX
GTX60874 Image
GTX60874 Image
GTX60874 Image
GTX60874 Image
GTX60874 Image
GTX60874 Image
The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
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The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.
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ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated. Antibody amount : 5µg
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ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Red : Primary antibody Green : Actin
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WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Dilution : 0.5µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | RM130 |
| Isotype: | IgG |
| Immunogen: | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) |
| Application: | ELISA, ICC, IF, ChIP, WB |
Additional Text
Modification
Acetylated, Lys14
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.5 µg/mL - 2 µg/mL. ICC/IF: 0.5 µg/mL - 2 µg/mL. ELISA: 0.2 µg/mL - 1 µg/mL. ChIP assay: 2 µg/mL - 10 µg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60874 Datasheet
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