Histone H3 antibody [RM188]
Catalogue Number: GTX60888-GTX
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The GTX60888 reacts to both Histone H3.1 and Histone H3.3.
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Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166] (2 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60886 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.
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Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody and biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.
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Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.
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Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody and GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.
GTX60888 Image
Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.
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ICC/IF analysis of HeLa cells using GTX60888 Histone H3 antibody [RM188]. Red : Primary antibody Green : Actin
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WB analysis of recombinant histone H3.3 and histone H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates using GTX60888 Histone H3 antibody [RM188]. Dilution : 0.025µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | RM188 |
| Isotype: | IgG |
| Immunogen: | A peptide corresponding to the C-terminus of human Histone H3. |
| Application: | ELISA, ICC, IF, sELISA, WB |
Additional Text
Gene ID
8350
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H3C1
Application Notes
WB: 0.01 µg/mL - 0.25 µg/mL. ICC/IF: 1 µg/mL - 5 µg/mL. ELISA: 0.2 µg/mL - 1 µg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX60888 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations