SOX2 antibody [GT1876]
Catalogue Number: GTX627404-GTX
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Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Green: GFAP antibody (GTX100850) diluted at 1:500. Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.
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SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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Non-transfected (-) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
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Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50
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Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 µg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057). Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10 % SDS-PAGE SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000
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Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE GTX627404 diluted at 1:5000
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SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis. A. 30 µg zebrafish eye lysate/extract 10 % SDS-PAGE SOX2 antibody [GT1876] (GTX627404) dilution: 1:500
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The data was published in the journal Int J Biol Sci in 2019.PMID: 31595152
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The data was published in the 2020 in Sci Rep. PMID: 32398775
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The data was published in the journal Sci Rep in 2016. PMID: 27344963
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The data was published in the journal Sci Rep in 2016. PMID: 27344963
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SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse hippocampus. SOX2 stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 34 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | SRY-box 2 , ANOP3 , MCOPS3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT1876 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr |
Description
Description: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]
Additional Text
Gene Name
SOX2
Gene ID
6657
Purification
Protein G purified
Uniprot ID
P48431
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
34
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:10000. IHC-Fr: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX627404 Datasheet
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