Catalogue Number: GTX627408-GTX
Sample (30 µg of whole cell lysate) A: Drosophila 10% SDS PAGE GTX627408 diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [GT239] (GTX627408) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10% SDS-PAGE GAPDH antibody [GT239] (GTX627408) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:500. Blue: Hoechst 33342 staining.
GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. GAPDH stained by GAPDH antibody [GT239] (GTX627408) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Manufacturer: | GeneTex |
Preservative: | no preservative|No Preservative |
Molecular Weight: | 36 |
Physical state: | Liquid |
Type: | Monoclonal Primary Antibody - Unconjugated |
Alias: | glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP |
Shipping Condition: | Blue Ice |
Unit(s): | 100 ul, 25 ul |
Host name: | Mouse |
Clone: | GT239 |
Isotype: | IgG2b |
Immunogen: | Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary. |
Application: | ICC, IF, IHC-P, WB, EMSA |
Description: The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
GAPDH
1 mg/ml
Protein G purified
2597
P04406
Monoclonal
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
36
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
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