Oct4 antibody [GT486]
Catalogue Number: GTX627419-GTX
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Oct4 antibody [GT486] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT486] dilution: 1:50
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Oct4 antibody detects Oct4 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX627419) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 µg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 µg of Oct4 antibody (GTX627419) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627419) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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Sample (10 µg of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant Oct4 protein 10% SDS PAGE GTX627419 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Oct4 antibody [GT486] detects Oct4 protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE (GTX627419) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 stained by Oct4 antibody [GT486] (GTX627419) diluted at 1:500. Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
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The data was published in the 2020 in Sci Rep. PMID: 32398775
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The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324
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Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using OCT3/4(GTX627419) antibody at 1:200 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (GTX627419) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 39 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT486 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, IP, WB |
Description
Description: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Additional Text
Gene ID
5460
Gene Name
POU5F1
Uniprot ID
Q01860
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FCM: 1:50-1:200. IP: 1:1000-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX627419 Datasheet
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