TET1 antibody [GT1462]
Catalogue Number: GTX627420-GTX
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TET1 antibody [GT1462] detects TET1 protein by western blot analysis. A. 50 µg whole cell lysate/extract from 293T cells transfected with scramble siRNA B. 50 µg whole cell lysate/extract from TET1-knockdowned 293T cells 6% SDS-PAGE TET1 antibody [GT1462] (GTX627420) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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NT2D1 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
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TET1 antibody [GT1462] detects TET1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TET1 stained by TET1 antibody [GT1462] (GTX627420) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TET1 antibody detects TET1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells 5 % SDS-PAGE TET1 antibody (GTX627420) dilution: 1:500
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The data was published in the journal Sci Rep in 2019.PMID: 31235851
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TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 235 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | GT1462 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Additional Text
Gene Name
Tet1
Uniprot ID
Q8NFU7
Purification
Protein G purified
Gene ID
80312
Molecular Weight
235
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX627420 Datasheet
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