Nanog antibody [GT3312]
Catalogue Number: GTX627421-GTX
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nanog antibody [GT3312] (GTX627421) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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NANOG antibody [GT3312] detects NANOG protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: NANOG antibody [GT3312] dilution: 1:50
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NANOG antibody [GT3312] detects NANOG protein by western blot analysis. A. 30 µg human ESC whole cell lysate/extract 10% SDS-PAGE NANOG antibody [GT3312] (GTX627421) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Sample (20 µg of whole cell lysate) A: mouse ESC 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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NANOG antibody [GT3312] validation by siRNA knock-down. Upperpanel: NANOG antibody [GT3312] GTX627421 Lower panel: GAPDH antibody (GTX100118) A. 30 µg Tera-2 whole cell lysate/extract B. 30 µg whole cell lysate/extract of NANOG siRNA#1-transfected Tera-2 cells C. 30 µg whole cell lysate/extract of NANOG siRNA#2-transfected Tera-2 cells D. 30 µg whole cell lysate/extract of NANOG siRNA#3-transfected Tera-2 cells 10% SDS-PAGE NANOG antibody [GT3312] (GTX627421) dilution: 1:1000 GAPDH antibody (GTX100118) dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX627421 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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The data was published in the journal Cancers (Basel) in 2019. PMID: 31878324
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The data was published in the journal Mol Cancer in 2013. PMID: 24094005
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Nanog homeobox |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT3312 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, WB |
Description
Description: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Additional Text
Gene Name
NANOG
Purification
Protein G purified
Gene ID
79923
Uniprot ID
Q9H9S0
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
35
Concentration
1 mg/ml
Short Description
Nanog antibody recognizes Nanog protein, a 35 kDa transcription factor that maintains pluripotency and the self-renewal feature of embryonic stem cells. It is one of the Thomson factors (i.e., NANOG, OCT4, SOX2, and LIN28) that induce a stable intrinsic pluripotency network to generate induced pluripotent stem cells. In addition, NANOG expression is elevated in cancer stem cells and correlates with a worse prognosis for many tumor types.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500-1:3000. FCM: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX627421 Datasheet
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