EasyBlot anti Rat IgG (HRP)
Catalogue Number: GTX628474-01-GTX
GTX628474-01 Image
GTX628474-01 Image
EasyBlot anti-Rat IgG (HRP) Comparison of western blot analysis with traditional secondary antibody (GTX224125-01 diluted at 1:10000) (1) and EasyBlot anti-Rat IgG kit (GTX628474-01 diluted at 1:2000) (2). A. 0.2 µg native rat IgG B. 0.1 µg native rat IgG C. 1 µg denatured rat IgG D. 2 µg denatured rat IgG E. 4 µg denatured rat IgG F. 8 µg denatured rat IgG
| Manufacturer: | GeneTex |
| Preservative: | 0.005% Thimerosal|0.005% Thimerosal |
| Physical state: | Liquid |
| Type: | Monoclonal Secondary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 ul, 50 ul |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | |
| Application: | WB |
Additional Text
Concentration
0.37 mg/ml
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
EasyBlot anti-Rat IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of Rat IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to rat IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian. To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Rat IgG (Optimized for Prot A/G) (GTX228909-01) or EasyBloker (GTX425858) to minimize protein A/G contamination.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628474-01 Datasheet
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