HIF1 alpha antibody [GT10211]
Catalogue Number: GTX628480-GTX
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HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500µM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
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HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments. IP Sample: 1000 µg HepG2 whole cell lysate/extract A. 40 µg HepG2 whole cell lysate/extract from normoxia B. 40 µg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) C. Control with 2.5 µg of preimmune mouse IgG/HepG2 whole cell lysate/extract from normoxia D. Control with 2.5 µg of preimmune mouse IgG/ HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) E. Immunoprecipitation of HIF-1 alpha protein by 2.5 µg of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from normoxia F. Immunoprecipitation of HIF-1 alpha protein by 2.5 µg of HIF-1 alpha antibody (GTX628480)/HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2) 10% SDS-PAGE The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
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HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr) 7.5% SDS-PAGE HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract (untreated) B. 30 µg HepG2 whole cell lysate/extract (500 µM CoCl2 treatment for 24 hr) 7.5 % SDS-PAGE HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:500
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Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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HIF1 alpha antibody [GT10211] detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha stained by HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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The data was published in the journal Nat Commun in 2016.PMID: 27094744
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The data was published in the journal Genome Biol in 2019.PMID: 30791937
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 93 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT10211 |
| Isotype: | IgG2a |
| Immunogen: | The immunogen used to generate this antibody corresponds to human HIF1 alpha |
| Application: | ICC, IF, IHC-P, IP, WB, IHC |
Description
Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq]
Additional Text
Gene Name
HIF1A
Gene ID
3091
Uniprot ID
Q16665
Purification
Protein G purified
Molecular Weight
93
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:100-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628480 Datasheet
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