IDH2 antibody [GT673]
Catalogue Number: GTX628487-GTX
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
GTX628487 Image
IDH2 antibody [GT673] detects IDH2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: IDH2 protein stained by IDH2 antibody [GT673] (GTX628487) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX628487 Image
Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628487 Image
IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. IDH2 antibody [GT673] (GTX628487) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX628487 Image
IDH2 antibody [GT673] detects IDH2 protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. IDH2 antibody [GT673] (GTX628487) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX628487 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDH2 antibody [GT673] (GTX628487) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628487 Image
IDH2 antibody detects IDH2 protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: IDH2 protein stained by IDH2 antibody (GTX628487) diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX628487 Image
IDH2 antibody [GT673] detects IDH2 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE IDH2 antibody [GT673] (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628487 Image
IDH2 antibody [GT673] detects IDH2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 12% SDS-PAGE IDH2 antibody [GT673] (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
GTX628487 Image
IDH2 antibody detects IDH2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg NCI-H929 whole cell lysate/extract 10% SDS-PAGE IDH2 antibody (GTX628487) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No Preservative |
| Molecular Weight: | 51 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | isocitrate dehydrogenase (NADP(+)) 2, mitochondrial , D2HGA2 , ICD-M , IDH , IDHM , IDP , IDPM , mNADP-IDH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT673 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human IDH2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. [provided by RefSeq]
Additional Text
Gene ID
3418
Gene Name
IDH2
Uniprot ID
P48735
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
51
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628487 Datasheet
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