alpha Tubulin antibody [GT114]
Catalogue Number: GTX628802-GTX
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alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract F. 30 µg drosophila lysae/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 µg zebrafish eye lysate/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunofluorescence photomicrographs of frozen sections of mouse brain. Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04). Blue: Nuclear staining with Hoechst 33342.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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The data was published in the 2018 in Sci Rep. PMID: 30459446
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The data was published in the journal PLoS One in 2014. PMID: 25000307
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The data was published in the journal Cell Biosci in 2019. PMID: 31044068
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The data was published in the journal Nat Commun in 2020. PMID: 32005813
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The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
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The data was published in the journal Toxins (Basel) in 2019. PMID: 31159487
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The data was published in the journal PLoS One in 2015. PMID: 25811469
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The data was published in the journal PLoS One in 2015. PMID: 25811469
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The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
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The data was published in the 2022 in Int J Mol Sci. PMID: 35055077
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The data was published in the 2022 in Int J Mol Sci. PMID: 35055077
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The data was published in the 2018 in Sci Rep. PMID: 30459446
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The data was published in the journal Nat Commun in 2016.PMID: 27221592
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The data was published in the journal PLoS Biol in 2019.PMID: 30883547
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The data was published in the journal Life Sci Alliance in 2019.PMID: 31767615
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The data was published in the journal Nat Commun in 2019.PMID: 31278365
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The data was published in the journal Genome Biol in 2018.PMID: 29548335
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The data was published in the journal Genome Biol in 2018.PMID: 29548335
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The data was published in the journal Biomolecules in 2020.PMID: 32635653
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The data was published in the journal Biomolecules in 2020.PMID: 32635653
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The data was published in the journal PLoS One in 2017.PMID: 28467493
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The data was published in the journal PLoS Biol in 2019.PMID: 30883547
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The data was published in the journal Physiol Rep in 2017.PMID: 28408640
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TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20oC 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
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alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | no Preservative|No Preservative |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Mouse |
| Clone: | GT114 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary. |
| Application: | FC, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Additional Text
Gene Name
TUBA1B
Gene ID
10376
Molecular Weight
50
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity
K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody
Short Description
alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX628802 Datasheet
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